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Plasmid list

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Plasmids

CMV-intron:
MLV moloney Gag Pol expression vector (packaging plasmid) From FL Cosset.

CNCG:
MLV vector encoding CMV NEO CMV GFP

HR-SIN CSGW PGK Puro:
HIV vector expressing GFP and puro from Lehner lab. Based on CSGW

EXN:
MLV vector encoding N-term double HA tag from Bieniasz Lab. See Zhang et al Virology 353:396-409.

pLai-GFP/Luc:
Full length HIV-1 ∆Env encoding GFP or Luc in place of Nef. See PMID 1683726.

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pCIG3N / PCIG3B:
N and B tropic MLV packaging plasmids. Described in Bock et al 2000 J Virol 74:7422-7430

SIV-LacZ:
SIVmac vector encoding LacZ from FL Cosset.

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SIV-GFP:
SIVmac vector encoding GFP from FL Cosset.

SIV3+:
SIVmac packaging plasmid encoding accessory genes from FL Cosset. Described in Negre et al Gene Therapy (2000) 7 pp1613-23

psvIII:
Deleted HIV-1 used to express HIV-1 Env (gp160) from Joe Sodroski.

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SVR:
HIV-2 molecular clone from Andrew Lever.

SVR∆NB GAG-POL∆ENV (HIV2-pack):
HIV-2 packaging plasmid encoding accessory genes. (We call it HIV-2 pack) from Andrew Lever.

HIV2-GFP / puro:
HIV-2 vector encoding either GFP or puro from Andrew Lever. Described in Griffin et al, J Virol, 2001 75(24):12058-69

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R9 BaL-env:
HIV Molecular clone R9 with PCR cloned macrophage tropic CCR5 using Env gene from HIV-1 Ba-L from Didier Trono

HIV-siren:
Varient of HIV vector CSGW with the shRNA expression cassette from BD commercial vector Siren RetroQ. Clone shRNA oligos between BamHI and EcoRI design shRNA using BD site.

pSIREN RETRO Q:
MLV shRNA vector

SFXUC:
HIV-1 vector derived from CSGW by David Escors. Clone into SFFV position using BamH1, Not1 or Sal1

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Towers Lab  -  Blizard Institute, Queen Mary University of London

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